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Objective:To investigate the clinical factors influencing the efficacy and prognosis of neoadjuvant chemoradiotherapy (nCRT) in patients with locally advanced rectal cancer (LARC).Methods:The clinical data of 335 patients with LARC who underwent radical operation after nCRT in Liaoning Cancer Hospital were retrospectively analyzed.Through univariate and multivariate analysis, we found out the clinical factors that may affect the efficacy and prognosis of nCRT in patients with LARC.Results:Multivariate logistic regression analysis showed that the independent clinical factors influencing the curative effect of nCRT in LARC patients were tumor invasion circumference( OR=2.350, 95% CI: 1.438-3.842, P=0.01), cT stage( OR=2.101, 95% CI: 1.024-4.314, P=0.043) and cN stage( OR=5.836, 95% CI: 3.305-10.306, P<0.01). Competitive risk model analysis showed that the independent factors affecting the prognosis of LARC patients were tumor regression grading (TRG) ( HR=3.236, 95% CI: 1.714-6.110, P<0.01), cT stage ( HR=1.852, 95% CI: 1.061-3.230, P=0.030), cN stage ( HR=2.008, 95% CI: 1.083-0.372, P=0.027), and carcinoembryonic antigen (CEA) level before nCRT ( HR=7.038, P<0.01), 95% CI: 3.894-12.720, P<0.01) and serum carbohydrate antigen 199 (CA199) ( HR=3.849, 95% CI: 2.016-7.350, P<0.01). Conclusion:The larger the circumference of tumor invasion before nCRT, cT stage and the cN stage were independent clinical factors influencing the nCRT efficacy of LARC patients.The larger the degree of tumor invasion to the rectum, the higher cT stage and the higher the cN stage before nCRT, the worse the efficacy of nCRT in LARC patients may be. TRG grade, cT stage, cN stage, CEA and CA199 level before nCRT were independent factors affecting the prognosis of LARC patients who received nCRT. The higher the TRG grade, of LARC patients receiving nCRT, the higher cT and cN stage, and the higher the CEA and CA199 level before nCRT, the worse the prognosis of LARC patients may be.
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Objective@#To investigate the clinical value of dual-phase arterial spin-labeled perfusion imaging(3D-ASL) in the evaluation of collateral circulation compensation in patients with severe cerebrovascular stenosis.@*Methods@#From December 2017 to August 2018, 73 patients with severe cerebral artery stenosis confirmed by MRA in Linfen Central Hospital were collected and studied.The two-phase superconducting magnetic resonance imaging was performed[Post Label Delay(PLD): 1 525ms, 2 525ms] three-dimensional quasli continuous arterial spin labeling(3D-PCASL) imaging.Bilateral phase cerebral blood flow(CBF) was measured on the affected side and mirror side respectively.The CBF values of the affected side and mirror side were compared and analyzed, and the abnormal perfusion cases were compared and analyzed.@*Results@#In 73 patients with severe cerebrovascular stenosis, the CBF values of the bilateral affected side[CBF 1 525ms(18.33±6.42)mL·100g-1·min-1, CBF 2 525ms(34.81±11.68)mL·100g-1·min-1] were lower than those of the mirror side[CBF 1 525ms(41.25±16.84)mL·100g-1·min-1, CBF 2 525ms(47.74±14.90)mL·100g-1·min-1], the differences were statistically significant(Z=-9.348, -7.476, all P<0.001). The ratio of abnormal perfusion in PLD=2525ms group[CBF(34.81±11.68)mL·100g-1·min-1]was higher than that in PLD=1 525ms group[CBF(18.33±6.42)mL·100g-1·min-1], the difference was statistically significant(Z=-9.196, P<0.001). The percentage of abnormal perfusion in PLD group of 2 525ms(56.2%) was lower than that in PLD group of 1 525ms(94.5%), the difference was statistically significant(χ2=5.393, P=0.02).@*Conclusion@#Dual-phase ASL(PLD=1 525ms, 2 525ms) combined with ASL can more accurately evaluate the compensations of fast collateral circulation and slow collateral circulation, and provide individualized treatment.
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Objective To investigate the clinical value of dual -phase arterial spin -labeled perfusion imaging(3D-ASL) in the evaluation of collateral circulation compensation in patients with severe cerebrovascular stenosis.Methods From December 2017 to August 2018,73 patients with severe cerebral artery stenosis confirmed by MRA in Linfen Central Hospital were collected and studied.The two-phase superconducting magnetic resonance imaging was performed[ Post Label Delay(PLD):1 525ms,2 525ms] three-dimensional quasli continuous arterial spin labeling(3D-PCASL) imaging.Bilateral phase cerebral blood flow (CBF) was measured on the affected side and mirror side respectively.The CBF values of the affected side and mirror side were compared and analyzed ,and the abnormal perfusion cases were compared and analyzed.Results In 73 patients with severe cerebrovascular stenosis , the CBF values of the bilateral affected side [ CBF 1 525ms (18.33 ±6.42) mL· 100g-1· min-1 ,CBF 2 525ms (34.81 ±11.68)mL· 100g-1· min-1 ] were lower than those of the mirror side [CBF 1 525ms(41.25 ±16.84)mL· 100g-1· min-1 ,CBF 2 525ms(47.74 ±14.90) mL· 100g-1· min-1 ],the differences were statistically significant (Z＝-9.348,-7.476,all P＜0.001).The ratio of abnormal perfusion in PLD ＝2525ms group[ CBF(34.81 ± 11.68)mL· 100g-1· min-1 ] was higher than that in PLD ＝1 525ms group[ CBF(18.33 ±6.42) mL· 100g-1· min-1 ],the difference was statistically significant (Z＝-9.196,P＜0.001).The percentage of abnormal perfusion in PLD group of 2 525ms(56.2%) was lower than that in PLD group of 1 525ms(94.5%),the difference was statisti- cally significant(χ2 ＝5.393,P＝0.02).Conclusion Dual-phase ASL( PLD＝1 525ms,2 525ms) combined with ASL can more accurately evaluate the compensations of fast collateral circulation and slow collateral circulation ,and provide individualized treatment.
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Objective To compare the effect of multislice spiral CT and 1.5T magnetic resonance imaging in the diagnosis of acute brain injury.Methods From June 2015 to February 2017,110 patients with suspected acute craniocerebral injury in Linfen Central Hospital were selected as study objects.All the patients received multi-slice spiral CT and 1.5T magnetic resonance imaging examination.The diagnostic value of two kinds of examination methods was compared.Results The accuracy rate,misdiagnosis rate,missed diagnosis rate,specificity and sensitivity of the magnetic resonance imaging were 95.00％,10.00％,5.00％,90.00％,95.00％,respectively,which of the multi-slice spiral CT were 58.00％,60.00％,42.00％,40.00％,58.00％,respectively,there were statistically significant differences between the two methods (x2 =38.075,5.494,98.075,5.494,38.075,P ＜ 0.05).Conclusion 1.5T magnetic resonance imaging for the diagnosis of acute craniocerebral injury is more significant.
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BACKGROUND:Hydroxyapatite/poly-L-lactic acid composite can wel improve the brittleness and mechanical properties of the hydroxyapatite. OBJECTIVE:To study the biocompatibility of hydroxyapatite/poly-L-lactic acid composite biomaterials with periodontal ligament cels. METHODS:Passage 4 human periodontal ligament cels at a density of 5×109/L were cultured with hydroxyapatite/poly-L-lactic acid composite as experimental group, and human periodontal ligament cels cultured alone as control group. Cel proliferation was detected within 7 days of culture; alkaline phosphatase activity was detected at 24, 48 and 72 hours of culture; and type I colagen expression was detected at 72 hours of culture. RESULTS AND CONCLUSION:There was no significant difference in the absorbance value of cels between the two groups at 1-7 days of culture. The alkaline phosphatase activity of cels at 48 and 72 hours was significantly higher than that at 24 hours (P < 0.05) in the two groups, but there was no difference between the two groups. The expression of type I colagen had no difference between the two groups. These findings indicate that the hydroxyapatite/poly-L-lactic acid composite biomaterial has good cel compatibility.
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<p><b>OBJECTIVE</b>To investigate the protective effect of hydrogen against hyperoxia-induced oxidative stress injury in premature rat type II alveolar epithelial cells (AECs).</p><p><b>METHODS</b>The type II AECs isolated from premature rats were randomly divided into air (21% oxygen) control group, hyperoxia (95% oxygen) control group, air + hydrogen group, and hyperoxia+ hydrogen group. The cells with hydrogen treatment were cultured in the presence of rich hydrogen. After the corresponding exposure for 24 h, the cell morphology was observed microscopically. MTT assay was used to evaluated the cell proliferation ability, and JC-1 fluorescence probe was used to detect the mitochondrial membrane potential (δφ) changes of the type II AECs. The concentration of maleic dialdehyde (MDA) and superoxide dismutase (SOD) activity in the cell supernatant were detected using colorimetric method.</p><p><b>RESULTS</b>No significant differences were found in cell growth or measurements between air control and air + hydrogen groups. Compared with air control group, the cells exposed to hyperoxia showed significantly suppressed proliferation, reduced mitochondrial membrane potential, increased MDA content, and decreased SOD activity. Intervention with hydrogen resulted in significantly increased cell proliferation and SOD activity and lowered MDA content, and restored the mitochondrial membrane potential in the cells with hyperoxia exposure (P<0.05).</p><p><b>CONCLUSION</b>Hydrogen can significantly reduce hyperoxia-induced oxidative stress injury in premature rat type II AECs, improve the cellular antioxidant capacity, stabilize the mitochondrial membrane potential, and reduce the inhibitory effect of hyperoxia on cell proliferation.</p>